In this publication we have performed CITE-seq analysis on total CD45⁺ cells isolated from the livers of mice fed a standard diet (SD) or western diet (WD; fat, cholesterol and sugar) for 12, 24 and 36 weeks. In addition, we also zoomed in on the monocyte and macrophage clusters in a separate UMAP to be able to more fully interrogate the data. We also performed single cell RNA sequencing analysis of the CD45^- cells isolated from the livers of the mice fed the SD or WD for 24 and 36 weeks. All datasets as presented in the publication are available here for interrogation. Genes and proteins can be searched and overall expression in the UMAPs as well as expression per cluster and in specific clusters over the course of the feeding can be displayed.

Osteopontin Expression Identifies a Subsetof Recruited Macrophages Distinctfrom Kupffer Cells in the Fatty Liver. Anneleen Remmerie, Liesbet Martens, Tinne Thoné, Angela Castoldi, Ruth Seurinck, Benjamin Pavie, Joris Roels, Bavo Vanneste, Sofie De Prijck, Mathias Vanhockerhout, Mushida Binte Abdul Latib, Lindsey Devisscher, Anne Hoorens, Johnny Bonnardel, Niels Vandamme, Anna Kremer, Peter Borghgraef, Hans Van Vlierberghe, Saskia Lippens, Edward Pearce, Yvan Saeys, and Charlotte L. Scott. Immunity, 2020.

https://www.cell.com/immunity/fulltext/S1074-7613(20)30357-5 Start using

The liver is the largest solid organ in the body, yet it remains incompletely characterized. Here, we present a spatial proteogenomic atlas of the healthy human and murine liver combining single-cell CITE-seq, single-nuclei sequencing, spatial transcriptomics and spatial proteomics. By integrating these multi-omic datasets, we provide validated strategies to reliably discriminate and localize all hepatic cells including a population of bile-duct lipid-associated macrophages (LAMs). We then align this atlas across seven species, revealing the conserved program of bona fide Kupffer cells and bile-duct LAMs. We also uncover the respective spatially-resolved cellular niches of these macrophages and the microenvironmental circuits driving their unique transcriptomic identities. We demonstrate that bile-duct LAMs are induced by local lipid exposure, leading to their induction in steatotic regions of the murine and human liver, while Kupffer cell development crucially depends on their crosstalk with hepatic stellate cells via the evolutionarily-conserved ALK1-BMP9/10 axis.

Spatial proteogenomics reveals distinct and evolutionarily conserved hepatic macrophage niches. Martin Guilliams, Johnny Bonnardel, Birthe Haest, Bart Vanderborght, Camille Wagner, Anneleen Remmerie, Anna Bujko, Liesbet Martens, Tinne Thoné, Robin Browaeys, Federico F. De Ponti, Bavo Vanneste, Christian Zwicker, Freya R. Svedberg, Tineke Vanhalewyn, Amanda Gonçalves, Saskia Lippens, Bert Devriendt, Eric Cox, Giuliano Ferrero, Valerie Wittamer, Andy Willaert, Suzanne J.F. Kaptein, Johan Neyts, Kai Dallmeier, Peter Geldhof, Stijn Casaert, Bart Deplancke, Peter ten Dijke, Anne Hoorens, Aude Vanlander, Frederik Berrevoet, Yves Van Nieuwenhove, Yvan Saeys, Wouter Saelens, Hans Van Vlierberghe, Lindsey Devisscher and Charlotte L. Scott. Cell, 2022.

https://www.cell.com/cell/fulltext/S0092-8674(21)01481-1 Start using

	Martin Guilliams, PhD
	Professor - Group leader
	Laboratory of Myeloid Cell Biology in Tissue Homeostasis and Regeneration
	VIB Inflammation Research Center (IRC)
	Universiteit Gent
	Technologiepark 71, 9052 Zwijnaarde, Belgium
	Tel: +32 933 13740
	martin.guilliams@irc.vib-ugent.be